CRISPR-Cas9- the future of genomic engineering?

The Biology Yak

It’s about time I did a post on CRISPR-Cas9! As always, words in pink will be added to the glossary

CRISPR-Cas9 is an up-and-coming method for gene modification, which has taken the scientific world by storm.

What is CRISPR?

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) are repetitive sections of DNA, associated with the Cas9 endonuclease.  It is commonly found as part of the bacterial immune system.

What does it do?

The CRISPR-Cas9 protein uses specific RNA guides to target and cleave very specific DNA. This means that CRISPR-Cas9 can be used for cutting highly targeted sites along the genome. The RNA guides the specificity, and can be artificially edited in vitro. CRISPR can knockout a gene of interest, and can also be used to cut out a gene, and replace it with a new one. In short, it can be used to edit a genome precisely, and this has had a massive impact on the…

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